DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00007064%3AK01__%2F17%3AN0000002" target="_blank" >RIV/00007064:K01__/17:N0000002 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >http://dx.doi.org/10.1080/24701394.2017.1298102</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >10.1080/24701394.2017.1298102</a>

Result language

angličtina

Original language name

DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Original language description

Several methods of DNA extraction, coupled with “DNA Barcode” species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages – egg, the first instar larvae, and empty pupal cases (puparia) – using four different extraction methods, namely, one simple "homemade" extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types – eggs, larvae, and pupari.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/VI20162020015" target="_blank" >VI20162020015: Introduction of New Methods of Human, Animal and Plant Material Identification in Forensic Practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mitochondrial DNA Part A

ISSN

2470-1408

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

21 Mar 2017

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

1-4

UT code for WoS article

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EID of the result in the Scopus database

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