DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77420" target="_blank" >RIV/60460709:41210/18:77420 - isvavai.cz</a>

Alternative codes found

RIV/00007064:K01__/18:N0000001 RIV/60077344:_____/18:00473646

Result on the web

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >http://dx.doi.org/10.1080/24701394.2017.1298102</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/24701394.2017.1298102" target="_blank" >10.1080/24701394.2017.1298102</a>

Result language

angličtina

Original language name

DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

Original language description

Several methods of DNA extraction, coupled with DNA barcoding species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages - eggs, the first instar larvae, and empty pupal cases (puparia) - using four different extraction methods, namely, one simple homemade extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The simple extraction buffer method was successful for half of the eggs and for the first instar larval samples. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and the DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective, the extraction was successful with regard to all sample types - eggs, larvae, and pupari

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mitochondrial DNA Part A

ISSN

2470-1394

e-ISSN

2470-1408

Volume of the periodical

29

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

427-430

UT code for WoS article

000435078900014

EID of the result in the Scopus database

2-s2.0-85015866868