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DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00007064%3AK01__%2F18%3AN0000001" target="_blank" >RIV/00007064:K01__/18:N0000001 - isvavai.cz</a>

  • Alternative codes found

    RIV/60077344:_____/18:00473646 RIV/60460709:41210/18:77420

  • Result on the web

    <a href="https://www.tandfonline.com/doi/abs/10.1080/24701394.2017.1298102?journalCode=imdn21" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/24701394.2017.1298102?journalCode=imdn21</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/https://doi.org/10.1080/24701394.2017.1298102" target="_blank" >https://doi.org/10.1080/24701394.2017.1298102</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    DNA extraction and barcode identification of development stages of forensically important flies in the Czech Republic

  • Original language description

    Several methods of DNA extraction, coupled with “DNA Barcode” species identification, were compared using specimens from early developmental stages of forensically important flies from the Calliphoridae and Sarcophagidae families. DNA was extracted at three immature stages – egg, the first instar larvae, and empty pupal cases (puparia) – using four different extraction methods, namely, one simple "homemade" extraction buffer protocol and three commercial kits. The extraction conditions, including the amount of proteinase K and incubation times, were optimized. The DNA Lego Kit and DEP-25 DNA Extraction Kit were useful for DNA extractions from the first instar larvae samples, and DNA Lego Kit was also successful regarding the extraction from eggs. The QIAamp DNA mini kit was the most effective; the extraction was successful with regard to all sample types – eggs, larvae, and pupari.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10603 - Genetics and heredity (medical genetics to be 3)

Result continuities

  • Project

    <a href="/en/project/VI20162020015" target="_blank" >VI20162020015: Introduction of New Methods of Human, Animal and Plant Material Identification in Forensic Practice</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis

  • ISSN

    2470-1394

  • e-ISSN

    2470-1408

  • Volume of the periodical

    29

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    4

  • Pages from-to

    427-430

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-85015866868