Assessment of mitochondrial DNA as an indicator of islet quality: an example in Goto Kakizaki rats

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F11%3A00002528" target="_blank" >RIV/00023001:_____/11:00002528 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/11:00370179
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0041134511013340" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0041134511013340</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.transproceed.2011.09.055" target="_blank" >10.1016/j.transproceed.2011.09.055</a>

Result language
angličtina
Original language name
Assessment of mitochondrial DNA as an indicator of islet quality: an example in Goto Kakizaki rats
Original language description
BACKGROUND: Diabetic Goto Kakizaki (GK) rats represent an established model of type 2 diabetes that exhibit an onset of pancreatic islet (PI) pathology characterized by islet hypertrophy with a decreased number of insulin-secreting ?-cells. Among the remaining ?-cells, oxidative phosphorylation (OXPHOS) and consequently glucose-stimulated insulin secretion (GSIS) are impaired, perhaps owing to a deficit in mitochondrial DNA (mtDNA). We sought to identify this abnormality. METHODS: ?-Cells were obtainedfrom Accutase-dissolved PI isolated from GK or Wistar rats and sorted based on the positive Zn(2+) signal of Newport Green. The mtDNA copy number per cell was quantified as the amplicon ratio by polymerase chain reaction using specific primers against the rat ND5 mt gene and UCP2 nuclear gene. RESULTS: The 12-month-old GK rats exhibited drastically reduced copy numbers per remaining ?-cell, from 7,400 ? 600 in 12-month old Wistar rats (100%) to 24 ? 4%; mtDNA content in heart and liver w
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
—

Project
<a href="/en/project/NS10219" target="_blank" >NS10219: Respiration, oxidative stress and mitochondrial morphology of Langerhans islets suited for transplantation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)