A novel nanoprobe for multimodal imaging is effectively incorporated into human melanoma metastatic cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F15%3A00059541" target="_blank" >RIV/00023001:_____/15:00059541 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/15:00447153 RIV/00216208:11110/15:10313087
Result on the web
<a href="http://www.mdpi.com/1422-0067/16/9/21658" target="_blank" >http://www.mdpi.com/1422-0067/16/9/21658</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms160921658" target="_blank" >10.3390/ijms160921658</a>
Alternative languages
Result language
angličtina
Original language name
A novel nanoprobe for multimodal imaging is effectively incorporated into human melanoma metastatic cell lines
Original language description
To facilitate efficient drug delivery to tumor tissue, several nanomaterials have been designed, with combined diagnostic and therapeutic properties. In this work, we carried out fundamental in vitro and in vivo experiments to assess the labeling efficacy of our novel theranostic nanoprobe, consisting of glycogen conjugated with a red fluorescent probe and gadolinium. Microscopy and resazurin viability assays were used to study cell labeling and cell viability in human metastatic melanoma cell lines. Fluorescence lifetime correlation spectroscopy (FLCS) was done to investigate nanoprobe stability. Magnetic resonance imaging (MRI) was performed to study T-1 relaxivity in vitro, and contrast enhancement in a subcutaneous in vivo tumor model. Efficient cell labeling was demonstrated, while cell viability, cell migration, and cell growth was not affected. FLCS showed that the nanoprobe did not degrade in blood plasma. MRI demonstrated that down to 750 cells/L of labeled cells in agar phantoms could be detected. In vivo MRI showed that contrast enhancement in tumors was comparable between Omniscan contrast agent and the nanoprobe. In conclusion, we demonstrate for the first time that a non-toxic glycogen-based nanoprobe may effectively visualize tumor cells and tissue, and, in future experiments, we will investigate its therapeutic potential by conjugating therapeutic compounds to the nanoprobe.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International journal of molecular sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
23
Pages from-to
21658-21680
UT code for WoS article
000364541000084
EID of the result in the Scopus database
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