Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F19%3APU134870" target="_blank" >RIV/00216305:26220/19:PU134870 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10400453 RIV/61989592:15110/19:73595150 RIV/61989592:15310/19:73595150
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0008622319305342" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0008622319305342</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carbon.2019.05.061" target="_blank" >10.1016/j.carbon.2019.05.061</a>
Alternative languages
Result language
angličtina
Original language name
Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells
Original language description
Tissue regeneration based on stem cell therapy is one of the most rapidly developing fields of modern medicine. Several properties of human mesenchymal stromal cells (MSCs), such as tropism toward a tumor or injury site, make them promising candidates for regenerative medicine, targeted therapy, or treating injured tissues. However, to fully understand the role of stem cells in therapeutic function, their visualization in vivo is essential. Here, we describe, for the first time, the use of biocompatible quaternized carbon dots (QCDs) as a novel stem-cell tracking probe for in vivo fluorescence imaging of transplanted human MSCs. By studying the in vitro cytotoxicity, intracellular distribution, and precise uptake mechanism, we showed that QCDs had a high biocompatibility and excellent fluorescence properties after 24 h incubation with MSCs. Further to demonstrate the in vivo feasibility of the system, QCD-labeled MSCs (100 μg/mL of QCDs, 24 h incubation time) were transplanted subcutaneously into an immunodeficient mouse and visualized by optical in vivo imaging. The labeled cells were strongly fluorescent, allowing their semi-quantitative detection. Moreover, the homing of intravenously transplanted QCD-labeled MSCs into the solid tumor was clearly shown. The results demonstrated that QCD-labeling of human MSCs is a highly promising approach for in vivo tracking during stem cell therapy.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CARBON
ISSN
0008-6223
e-ISSN
1873-3891
Volume of the periodical
152
Issue of the periodical within the volume
2019
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
434-443
UT code for WoS article
000483384900047
EID of the result in the Scopus database
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