Characterisation and comparison of adipose tissue macrophages from human subcutaneous, visceral and perivascular adipose tissue

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F16%3A00059981" target="_blank" >RIV/00023001:_____/16:00059981 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10324848

Result on the web

<a href="http://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0962-1" target="_blank" >http://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0962-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12967-016-0962-1" target="_blank" >10.1186/s12967-016-0962-1</a>

Result language

angličtina

Original language name

Characterisation and comparison of adipose tissue macrophages from human subcutaneous, visceral and perivascular adipose tissue

Original language description

Background and aims: Macrophages play important roles in adipose tissue inflammation and its consequences. Unfortunately, a detailed description of the macrophage phenotypes in different human adipose tissues is not available. Subjects and methods: Subcutaneous, visceral and perivascular adipose tissues were obtained from 52 living kidney donors during live donor nephrectomy. Stromal vascular fractions were isolated, and the macrophage phenotypes were analyzed by flow cytometry using surface markers (CD14, CD16, CD36, and CD163). Results: In addition to CD16 positivity, pro-inflammatory macrophages also display high scavenger receptor CD36 expression. The great majority of CD16 negative macrophages express the anti-inflammatory CD163 marker. The presence of pro-inflammatory macrophages was almost twice as high in visceral (p < 0.0001) and perivascular (p < 0.0001) adipose tissues than in subcutaneous tissue. This difference was substantially more pronounced in the postmenopausal women subgroup, consequentlly, the total difference was driven by this subgroup. Conclusion: We obtained detailed information about M1 and M2 macrophage phenotypes in human adipose tissue. The visceral and perivascular adipose tissues had substantially higher pro-inflammatory characteristics than the subcutaneous tissue. The higher proportion of pro-inflammatory macrophages in the visceral adipose tissue of postmenopausal women might be related to an increased cardiovascular risk.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of translational medicine [online]

ISSN

1479-5876

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

July 2016

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

"art. no. 208"

UT code for WoS article

000379809600001

EID of the result in the Scopus database

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