Pre-microporation mproves outcome of pancreatic islet labelling for optical and F-19 MR imaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F17%3A00075977" target="_blank" >RIV/00023001:_____/17:00075977 - isvavai.cz</a>
Result on the web
<a href="https://biologicalproceduresonline.biomedcentral.com/track/pdf/10.1186/s12575-017-0055-4?site=biologicalproceduresonline.biomedcentral.com" target="_blank" >https://biologicalproceduresonline.biomedcentral.com/track/pdf/10.1186/s12575-017-0055-4?site=biologicalproceduresonline.biomedcentral.com</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12575-017-0055-4" target="_blank" >10.1186/s12575-017-0055-4</a>
Alternative languages
Result language
angličtina
Original language name
Pre-microporation mproves outcome of pancreatic islet labelling for optical and F-19 MR imaging
Original language description
Background: In vitro labelling of cells and small cell structures is a necessary step before in vivo monitoring of grafts. We modified and optimised a procedure for pancreatic islet labelling using bimodal positively charged poly(lactic-coglycolic acid) nanoparticles with encapsulated perfluoro crown ethers and indocyanine green dye via microporation and compared the method with passive endocytosis. Results: Pancreatic islets were microporated using two pulses at various voltages. We tested a standard procedure (poration in the presence of nanoparticles) and a modified protocol (pre-microporation in a buffer only, and subsequent islet incubation with nanoparticles on ice for 10 min). We compared islet labelling by microporation with labelling by endocytosis, i.e. pancreatic islets were incubated for 24 h in a medium with suspended nanoparticles. In order to verify the efficiency of the labelling procedures, we used F-19 magnetic resonance imaging, optical fluorescence imaging and confocal microscopy. The experiment confirmed that microporation, albeit fast and effective, is invasive and may cause substantial harm to islets. To achieve sufficient poration and to minimise the reduction of viability, the electric field should be set at 20 kV/m (two pulses, 20 ms each). Poration in the presence of nanoparticles was found to be unsuitable for the nanoparticles used. The water suspension of nanoparticles (which served as a surfactant) was slightly foamy and microbubbles in the suspension were responsible for sparks causing the destruction of islets during poration. However, pre-microporation (poration of islets in a buffer only) followed by 10-min incubation with nanoparticles was safer.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biological procedures online
ISSN
1480-9222
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
June 28
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
"art. no. 6"
UT code for WoS article
000405852100001
EID of the result in the Scopus database
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