Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7T

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F16%3A00060053" target="_blank" >RIV/00023001:_____/16:00060053 - isvavai.cz</a>

Result on the web

<a href="http://www.omicsonline.org/open-access/magnetic-resonance-visualization-of-pancreatic-islets-labeled-by-paracest-contrast-agents-at-47-t-2155-9937-1000121.php?aid=68387" target="_blank" >http://www.omicsonline.org/open-access/magnetic-resonance-visualization-of-pancreatic-islets-labeled-by-paracest-contrast-agents-at-47-t-2155-9937-1000121.php?aid=68387</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4172/2155-9937.1000121" target="_blank" >10.4172/2155-9937.1000121</a>

Result language

angličtina

Original language name

Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7T

Original language description

Introduction: Monitoring of Pancreatic Islets (PIs) after their transplantation could provide important data related to diabetes treatment; however their visualization is conditioned by the contrast agent use. The aim of this study was to label and visualize PIs labeled by the contrast agents based on Chemical Exchange Saturation Transfer (CEST) owing an advantage of contrast switching on/off and simultaneous visualization of differently treated cell populations in one MR experiment. Material and methods: Two paramagnetic chelates with europium Eu-DO3A-ae and ytterbium Yb-DO3A-ae were tested for labeling of PIs by pinocytosis and microporation. The toxicity, labeling efficacy and detection threshold were assessed for each agent. In vitro MR imaging of the labeled islets was performed on a 4.7 T scanner using a modified turbo spin-echo (RARE) sequence. Results: The sufficient labeling efficacy was observed only by using high agent concentration compromising islet viability. The microporation procedure was not effective for islet labeling because of its invasive nature. The islets labeled by pinocytosis were visualized in a phantom by CEST imaging, however, after a long acquisition time at 4.7 T. Conclusion: The low sensitivity of detection and impaired cellular viability of the pancreatic islets labeled by the novel CEST contrast agents represent a challenge for the further implementation of these probes as exogenous cellular labels.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Molecular Imaging and Dynamics

ISSN

2155-9937

e-ISSN

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Volume of the periodical

2016

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

1-7

UT code for WoS article

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EID of the result in the Scopus database

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