Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F17%3A00076179" target="_blank" >RIV/00023001:_____/17:00076179 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Original language description

Inflammatory changes, both in the arterial wall and adipose tissue, play a crucial role in the development of atherosclerosis. We measured the gene expression of tumor necrosis factor-alpha (TNF alpha), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living kidney donors (LKD) and patients with peripheral arterial disease (PAD). Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue (PVAT). Data of PAD patients showed significantly higher expression in VAT in all three genes (TNFa 5-fold, p&lt;0.05; MCP-1 3.6-fold, p&lt;0.05; IL-6 18.8-fold, p&lt;0.001). The differences in PVAT and SAT were less significant. Total body pro-inflammatory status was documented by higher TNFa concentration in patients (4.86 +/- 1.4 pg/ml) compared to LKDs (2.14 +/- 0.9 pg/ml; p&lt;0.001), as was hsCRP (11.8 +/- 7.0 in PAD; 1.5 +/- 0.48 in LKDs; p=0.017). We found no age-dependent relationship between gene expression vs. TNFa and hsCRP concentrations in both compared groups. No effect of the atherosclerosis score on gene expression and circulating inflammatory markers within the PAD group was observed. Our results suggest that the AT of PAD patients infiltrated with macrophages produces more cytokines involved in the development of inflammation and atherosclerosis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

66

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

633-640

UT code for WoS article

000412736000009

EID of the result in the Scopus database

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