Decellularized pancreatic tail as matrix for pancreatic islet transplantation into the greater omentum in rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F22%3A00083495" target="_blank" >RIV/00023001:_____/22:00083495 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/22:00565357 RIV/46747885:24530/22:00010469 RIV/00216208:11110/22:10452458

Result on the web

<a href="https://www.mdpi.com/2079-4983/13/4/171" target="_blank" >https://www.mdpi.com/2079-4983/13/4/171</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jfb13040171" target="_blank" >10.3390/jfb13040171</a>

Result language

angličtina

Original language name

Decellularized pancreatic tail as matrix for pancreatic islet transplantation into the greater omentum in rats

Original language description

Infusing pancreatic islets into the portal vein currently represents the preferred approach for islet transplantation, despite considerable loss of islet mass almost immediately after implantation. Therefore, approaches that obviate direct intravascular placement are urgently needed. A promising candidate for extrahepatic placement is the omentum. We aimed to develop an extracellular matrix skeleton from the native pancreas that could provide a microenvironment for islet survival in an omental flap. To that end, we compared different decellularization approaches, including perfusion through the pancreatic duct, gastric artery, portal vein, and a novel method through the splenic vein. Decellularized skeletons were compared for size, residual DNA content, protein composition, histology, electron microscopy, and MR imaging after repopulation with isolated islets. Compared to the other approaches, pancreatic perfusion via the splenic vein provided smaller extracellular matrix skeletons, which facilitated transplantation into the omentum, without compromising other requirements, such as the complete depletion of cellular components and the preservation of pancreatic extracellular proteins. Repeated MR imaging of iron-oxide-labeled pancreatic islets showed that islets maintained their position in vivo for 49 days. Advanced environmental scanning electron microscopy demonstrated that islets remained integrated with the pancreatic skeleton. This novel approach represents a proof-of-concept for long-term transplantation experiments.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20601 - Medical engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of functional biomaterials

ISSN

2079-4983

e-ISSN

2079-4983

Volume of the periodical

13

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

17

Pages from-to

"art. no. 171"

UT code for WoS article

000901294600001

EID of the result in the Scopus database

2-s2.0-85144872942