Macrocomplexes: an establishing critical limits of post-PEG serum enzymes and proteins in adults and children
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F25%3A00085559" target="_blank" >RIV/00023001:_____/25:00085559 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/25:43928378
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0009898125002050?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0009898125002050?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cca.2025.120326" target="_blank" >10.1016/j.cca.2025.120326</a>
Alternative languages
Result language
angličtina
Original language name
Macrocomplexes: an establishing critical limits of post-PEG serum enzymes and proteins in adults and children
Original language description
Background: Exclusion of serum macroform presence is essential to avoid potential misdiagnosis. The aim of this study was to determine critical limits (CL) for enzymes (total and pancreatic amylase, AMY and pAMY; aminotransferases, AST and ALT; alkaline phosphatase, ALP; creatine kinase, CK; lipase, LIP; gamma-glutamyl transferase, GGT; lactate dehydrogenase, LDH) after polyethylene glycol (PEG) precipitation in children and adults. Methods: A total of 126 sera of patients suspected for macroenzyme presence (adults or children) was matched by serum enzyme activity of controls and all these sera were precipitated by PEG. PEG-precipitable activity ( %PPA) and CLs of %PPA were calculated for adults, children, and controls. Results: CLs of %PPA were substantially lower than common 60 % for ALP and CK in all subjects. Significant differences between adults and children were found for ALT and ALP (lower in children), and LIP and CK (higher in children). No meaningful correlation was found between initial enzyme activity and %PPA. Conclusions: Age specific CLs should be used for ALP, ALT, CK, and LIP. Generally accepted value of %PPA of 60 % may be used with caution for AMY, pAMY, AST, GGT, and LDH. A careful selection of CLs is needed for timely detection of macrocomplexes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])
Result continuities
Project
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Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinica chimica acta
ISSN
0009-8981
e-ISSN
1873-3492
Volume of the periodical
574
Issue of the periodical within the volume
15 June 2025
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
"art. no. 120326"
UT code for WoS article
001481468600001
EID of the result in the Scopus database
2-s2.0-105003238359