Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F11%3A00009312" target="_blank" >RIV/00023736:_____/11:00009312 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jcb.23263" target="_blank" >http://dx.doi.org/10.1002/jcb.23263</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcb.23263" target="_blank" >10.1002/jcb.23263</a>

Result language
angličtina
Original language name
Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes
Original language description
The effects of the pan-caspase inhibitor Q-VD-OPh on caspase activity, DNA fragmentation, PARP cleavage, 7A6 exposition, and cellular adhesivity to fibronectin were analyzed in three different apoptotic systems involving two cell lines and two apoptosisinducers. Q-VD-OPh fully inhibited caspase-3 and -7 activity at 0.05 ?M concentration, but significantly higher Q-VD-OPh dose (10 ?M) was required to prevent the cleavage of PARP-1. DNA fragmentation and cell membrane permeabilization were both preventedat 2 ?M Q-VD-OPh while 10 ?M inhibitor was needed to inhibit the loss of cellular adhesivity to fibronectin. The exposition of the mitochondrial antigen 7A6 occurred independently of Q-VD-OPh addition and may serve to the detection of cumulative incidence of the cells which have initiated the apoptosis. Q-VD-OPh efficiency in the whole-cell environment is of two orders of magnitude higher than that of z-VAD-fmk and this is not due to a low permeability of the latter through the membrane
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Project
<a href="/en/project/GA301%2F09%2F1026" target="_blank" >GA301/09/1026: Proteomic analysis of suberoylanilide hydroxamic acid (SAHA) effects on human leukemic cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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