Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F16%3A00011564" target="_blank" >RIV/00023736:_____/16:00011564 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/16:00463754

Result on the web

<a href="http://dx.doi.org/10.1039/C6AY01833E" target="_blank" >http://dx.doi.org/10.1039/C6AY01833E</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C6AY01833E" target="_blank" >10.1039/C6AY01833E</a>

Result language

angličtina

Original language name

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Original language description

Nonspecific protein adsorption, referred to as fouling, is a major clinical problem accompanying any material coming into direct contact with biological fluids, especially blood/plasma. Not only is protein fouling detrimental per se but it also promotes platelet adhesion, the initiation of blood coagulation, and/or complement activation. In spite of efforts to develop novel surfaces which suppress protein fouling, still only little is known about the mechanisms behind protein fouling from blood and other complex biological media. In order to unravel the mechanisms, precise identification of the proteins that foul the surface is necessary. This is particularly challenging for those surfaces with reduced protein fouling, such as polymer brushes. This report introduces a route to analyze the proteins by mass spectrometry using an optimized protocol. We demonstrate proteomics-compatible buffers/solutions suitable for desorption of adsorbed proteins on low fouling polymer brushes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical methods

ISSN

1759-9660

e-ISSN

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Volume of the periodical

34

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

6415-6419

UT code for WoS article

000382118500005

EID of the result in the Scopus database

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