NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F21%3A00013257" target="_blank" >RIV/00023736:_____/21:00013257 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/21:00554898 RIV/00159816:_____/21:00075079 RIV/00216224:14110/21:00122134
Result on the web
<a href="https://doi.org/10.1016/j.isci.2021.102683" target="_blank" >https://doi.org/10.1016/j.isci.2021.102683</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.isci.2021.102683" target="_blank" >10.1016/j.isci.2021.102683</a>

Result language
angličtina
Original language name
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses
Original language description
Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being tested as a treatment for graft-versus-host disease (GvHD). The immunosuppressive properties of MSCs seem beneficial, however, their response during fungal infection, which is an important cause of mortality in patients with GvHD, is unknown. We report that MSCs phagocytose the fungal component zymosan, resulting in phosphorylation of spleen tyrosine kinase (Syk), increase in cytosolic calcium levels, and ultimately, increase in NFAT1 nuclear translocation. RNA sequencing analysis of zymosan-treated MSCs showed that CN-NFAT inhibition affects extracellular matrix (ECM) genes but not cytokine expression that is under the control of the NF-kappa B pathway. When coculturing MSCs or decellularized MSC-ECM with human peripheral blood mononuclear cells (PBMCs), selective NFAT inhibition in MSCs decreased cytokine expression by PBMCs. These findings reveal a dual mechanism underlying the MSC response to zymosan: while NF-kappa B directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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