Calcineurin-NFAT signaling controls neutrophils' ability of chemoattraction upon fungal infection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F24%3A00013728" target="_blank" >RIV/00023736:_____/24:00013728 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/24:00139184 RIV/00159816:_____/24:00081026

Result on the web

<a href="https://doi.org/10.1093/jleuko/qiae091" target="_blank" >https://doi.org/10.1093/jleuko/qiae091</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jleuko/qiae091" target="_blank" >10.1093/jleuko/qiae091</a>

Result language

angličtina

Original language name

Calcineurin-NFAT signaling controls neutrophils' ability of chemoattraction upon fungal infection

Original language description

Calcineurin-nuclear factor of activated T cells (CN-NFAT) inhibitors are widely clinically used drugs for immunosuppression, but besides their required T cell response inhibition, they also undesirably affect innate immune cells. Disruption of innate immune cell function can explain the observed susceptibility of CN-NFAT inhibitor-treated patients to opportunistic fungal infections. Neutrophils play an essential role in innate immunity as a defense against pathogens, however, the effect of CN-NFAT inhibitors on neutrophil function was poorly described. Thus, we tested the response of human neutrophils to opportunistic fungal pathogens, namely Candida albicans and Aspergillus fumigatus, in the presence of CN-NFAT inhibitors. Here, we report that the NFAT pathway members were expressed in neutrophils and mediated part of the neutrophil response to pathogens. Upon pathogen exposure, neutrophils underwent profound transcriptomic changes with subsequent production of effector molecules. Importantly, genes and proteins involved in the regulation of the immune response and chemotaxis, including the chemokines CCL2, CCL3, and CCL4 were significantly upregulated. The presence of CN-NFAT inhibitors attenuated the expression of these chemokines and impaired the ability of neutrophils to chemoattract other immune cells. Our results amend knowledge about the impact of CN-NFAT inhibition in human neutrophils.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30205 - Hematology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of leukocyte biology

ISSN

0741-5400

e-ISSN

—

Volume of the periodical

116

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

816-829

UT code for WoS article

001217258900001

EID of the result in the Scopus database

2-s2.0-85202912440