Cytoplasmic localization of Mdm2 in cells expressing mutated NPM is mediated by p53
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F23%3A00013539" target="_blank" >RIV/00023736:_____/23:00013539 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/23:10474178
Result on the web
<a href="https://doi.org/10.1111/febs.16810" target="_blank" >https://doi.org/10.1111/febs.16810</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/febs.16810" target="_blank" >10.1111/febs.16810</a>
Alternative languages
Result language
angličtina
Original language name
Cytoplasmic localization of Mdm2 in cells expressing mutated NPM is mediated by p53
Original language description
Specific C-terminal nucleophosmin (NPM) mutations are related to the acute myeloid leukaemia and cause mistargeting of mutated NPM (NPMmut) to the cytoplasm. Consequently, multiple NPM-interacting partners, e.g., the tumour suppressor p53, become also mislocalized. We found that ubiquitin ligase Mdm2 mislocalizes to the cytoplasm in the presence of NPMmut as well. Since p53 interacts with Mdm2, we searched for the NPMmut-p53-Mdm2 complex and interactions of its constituents in live cells and cell lysates using fluorescently tagged proteins, fluorescence lifetime imaging and immunoprecipitation. We proved existence of the ternary complex, which likely adopts a chain-like configuration. Interaction between Mdm2 and NPMmut was not detected, even under conditions of upregulated Mdm2 and p53 induced by Actinomycin D.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/GA22-03875S" target="_blank" >GA22-03875S: Role of acute myeloid leukemia-associated nucleophosmin mutations in the NPM-p53-Mdm2 regulatory network</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEBS journal
ISSN
1742-464X
e-ISSN
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Volume of the periodical
290
Issue of the periodical within the volume
17
Country of publishing house
GB - UNITED KINGDOM
Number of pages
19
Pages from-to
4281-4299
UT code for WoS article
000985918100001
EID of the result in the Scopus database
2-s2.0-85159069739