AML-related NPM mutations drive p53 delocalization into the cytoplasm with possible impact on p53-dependent stress response
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F21%3A00013253" target="_blank" >RIV/00023736:_____/21:00013253 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/21:10434474
Result on the web
<a href="https://doi.org/10.3390/cancers13133266" target="_blank" >https://doi.org/10.3390/cancers13133266</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cancers13133266" target="_blank" >10.3390/cancers13133266</a>
Alternative languages
Result language
angličtina
Original language name
AML-related NPM mutations drive p53 delocalization into the cytoplasm with possible impact on p53-dependent stress response
Original language description
We investigated p53-NPM interaction in live HEK-293T cells by FLIM-FRET and in cell lysates by immunoprecipitation. eGFP lifetime-photoconversion was used to follow redistribution dynamics of NPMmut and p53 in Selinexor-treated cells. We confirmed the p53-NPMwt interaction in intact cells and newly documented that this interaction is not compromised by the NPM mutation causing displacement of p53 to the cytoplasm. Moreover, the interaction was not abolished for non-oligomerizing NPM variants with truncated oligomerization domain, suggesting that oligomeriza-tion is not essential for interaction of NPM forms with p53. Inhibition of the nuclear exporter XPO1 by Selinexor caused expected nuclear relocalization of both NPMmut and p53. However, significantly different return rates of these proteins indicate nontrivial mechanism of p53 and NPMmut cellular trafficking. The altered p53 regulation in cells expressing NPMmut offers improved understanding to help investigational strategies targeting these mutations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/GA19-04099S" target="_blank" >GA19-04099S: Role of nucleophosmin interactome in acute myeloid leukemia with mutated NPM</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cancers
ISSN
2072-6694
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
13
Country of publishing house
CH - SWITZERLAND
Number of pages
24
Pages from-to
"art. no. 3266"
UT code for WoS article
000671357300001
EID of the result in the Scopus database
2-s2.0-85108738984