A functional assay for the determination of heparin-induced thrombocytopenia via flow cytometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F23%3A00013570" target="_blank" >RIV/00023736:_____/23:00013570 - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/23:E0110427
Result on the web
<a href="https://doi.org/10.3390/diagnostics13183019" target="_blank" >https://doi.org/10.3390/diagnostics13183019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/diagnostics13183019" target="_blank" >10.3390/diagnostics13183019</a>
Alternative languages
Result language
angličtina
Original language name
A functional assay for the determination of heparin-induced thrombocytopenia via flow cytometry
Original language description
Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT IgG, ZYMUTEST HIA Monostrip IgG) to detect the presence of antibodies against heparin–PF4 complexes (platelet factor 4). When the immunoassay was positive, the sample was subsequently analyzed for HIT with a functional flow cytometry assay, the HITAlert kit, the purpose of which was to demonstrate the ability of the antibodies present to activate platelets. A diagnosis of HIT can be made only after a positive functional test result. In this article, we present an overview of our practical experience with the use of the new functional method of analysis, HIT, with flow cytometry. In this work, we compared the mutual sensitivity of two functional tests, SRA and the flow cytometry HITAlert kit, in patients perceived as being at risk for HIT. This work aims to delineate the principle, procedure, advantages, pitfalls, and possibilities of the application of the functional test HITAlert using flow cytometry.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Diagnostics
ISSN
2075-4418
e-ISSN
—
Volume of the periodical
13
Issue of the periodical within the volume
18
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
"art. no. 3019"
UT code for WoS article
001073307400001
EID of the result in the Scopus database
2-s2.0-85172227086