A Comprehensive Evaluation of Steroid Metabolism in Women with Intrahepatic Cholestasis of Pregnancy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F16%3AN0000017" target="_blank" >RIV/00023761:_____/16:N0000017 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10326629 RIV/00064165:_____/16:10326629

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A Comprehensive Evaluation of Steroid Metabolism in Women with Intrahepatic Cholestasis of Pregnancy

Original language description

Intrahepatic cholestasis of pregnancy (ICP) is a common liver disorder, mostly occurring in the third trimester. ICP is defined as an elevation of serum bile acids, typically accompanied by pruritus and elevated activities of liver aminotransferases. ICP is caused by impaired biliary lipid secretion, in which endogenous steroids may play a key role. Although ICP is benign for the pregnant woman, it may be harmful for the fetus. We evaluated the differences between maternal circulating steroids measured by RIA (17-hydroxypregnenolone and its sulfate, 17-hydroxyprogesterone, and cortisol) and GC-MS (additional steroids), hepatic aminotransferases and bilirubin in women with ICP (n = 15, total bile acids (TBA) > 8 mu M) and corresponding controls (n = 17). An age-adjusted linear model, receiver-operating characteristics (ROC), and multivariate regression (a method of orthogonal projections to latent structure, OPLS) were used for data evaluation. While aminotransferases, conjugates of pregnanediols, 17-hydroxypregnenolone and 5 beta-androstane-3 alpha, 17 beta-diol were higher in ICP patients, 20 alpha-dihydropregnenolone, 16 alpha-hydroxy-steroids, sulfated 17-oxo-C19-steroids, and 5 beta-reduced steroids were lower. The OPLS model including steroids measured by GC-MS and RIA showed 93.3% sensitivity and 100% specificity, while the model including steroids measured by GC-MS in a single sample aliquot showed 93.3% sensitivity and 94.1% specificity. A composite index including ratios of sulfated 3 alpha/beta-hydroxy-5 alpha/beta-androstane-17-ones to conjugated 5 alpha/beta-pregnane-3 alpha/beta, 20 alpha-diols discriminated with 93.3% specificity and 81.3% sensitivity (ROC analysis). These new data demonstrating altered steroidogenesis in ICP patients offer more detailed pathophysiological insights into the role of steroids in the development of ICP.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

<a href="/en/project/NT12211" target="_blank" >NT12211: Prediction of termination of pregnancy in women with serious hepatopathies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PloS One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

24

Pages from-to

nestrankovano

UT code for WoS article

000381369500005

EID of the result in the Scopus database

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