A Method for Determination of One Hundred Endogenous Steroids in Human Serum by Gas Chromatography-Tandem Mass Spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023761%3A_____%2F19%3AN0000027" target="_blank" >RIV/00023761:_____/19:N0000027 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10397385 RIV/00064165:_____/19:10397385
Result on the web
<a href="http://www.biomed.cas.cz/physiolres/pdf/2019/68_179.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/2019/68_179.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934124" target="_blank" >10.33549/physiolres.934124</a>
Alternative languages
Result language
angličtina
Original language name
A Method for Determination of One Hundred Endogenous Steroids in Human Serum by Gas Chromatography-Tandem Mass Spectrometry
Original language description
Steroid profiling helps various pathologies to be rapidly diagnosed. Results from analyses investigating steroidogenic pathways may be used as a tool for uncovering pathology causations and proposals of new therapeutic approaches. The purpose of this study was to address still underutilized application of the advanced GC-MS/MS platform for the multicomponent quantification of endogenous steroids. We developed and validated a GC-MS/MS method for the quantification of 58 unconjugated steroids and 42 polar conjugates of steroids (after hydrolysis) in human blood. The present method was validated not only for blood of men and non-pregnant women but also for blood of pregnant women and for mixed umbilical cord blood. The spectrum of analytes includes common hormones operating via nuclear receptors as well as other bioactive substances like immunomodulatory and neuroactive steroids. Our present results are comparable with those from our previously published GC-MS method as well as the results of others. The present method was extended for corticoids and 17 alpha-hydroxylated 5 alpha/beta-reduced pregnanes, which are useful for the investigation of alternative "backdoor" pathway. When comparing the analytical characteristics of the present and previous method, the first exhibit by far higher selectivity, and generally generally higher sensitivity and better precision particularly for 17 alpha-hydroxysteroids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/NV17-30528A" target="_blank" >NV17-30528A: Prediction of gestational diabetes on the basis of steroid metabolism</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PHYSIOLOGICAL RESEARCH
ISSN
0862-8408
e-ISSN
1802-9973
Volume of the periodical
68
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
29
Pages from-to
179-207
UT code for WoS article
000465948800006
EID of the result in the Scopus database
2-s2.0-85065492017