Induction of nonmucoid variants of Clavibacter michiganensis subsp. sepedonicus and comparison of their immunochemical and biochemical characteristics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F02%3A925" target="_blank" >RIV/00027006:_____/02:925 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Induction of nonmucoid variants of Clavibacter michiganensis subsp. sepedonicus and comparison of their immunochemical and biochemical characteristics

Original language description

A total of 38 mucoid strains of Clavibacter michiganensis subsp. sepedonicus (Cms) were grown on culture media under different conditions e.g., of sugar concentration and temperatures with the aim to specify factors, which induce or stimulate a change ofcolony morphology from the mucoid wild type to nonmucoid form. It was found that stress conditions during cultivation, i. e. temperatures above 28°C (29-31°C), and culture media containing lower quantity of sugars, i.e. less than recommended level, stimulated the changes in colony morphology. The degree of mucoid growth of Cms strains decreased under the influence of these stress factors. Nevertheless, the influence of tested factors was not sufficient to change mucoid strains irreversibly to nonmucoidones. Partially nonmucoid variants were termed "intermediate" in colony morphology. Using DAS-ELISA and Biolog GP MicroPlate SystemTM, mucoid and intermediate variants of Cms were tested to see if these techniques might serve to identify

Czech name

Indukce nemukoidních variant Clavibacter michiganensis subsp. sepedonicus

Czech description

A total of 38 mucoid strains of Clavibacter michiganensis subsp. sepedonicus (Cms) were grown on culture media under different conditions e.g., of sugar concentration and temperatures with the aim to specify factors, which induce or stimulate a change ofcolony morphology from the mucoid wild type to nonmucoid form. It was found that stress conditions during cultivation, i. e. temperatures above 28°C (29-31°C), and culture media containing lower quantity of sugars, i.e. less than recommended level, stimulated the changes in colony morphology. The degree of mucoid growth of Cms strains decreased under the influence of these stress factors. Nevertheless, the influence of tested factors was not sufficient to change mucoid strains irreversibly to nonmucoidones. Partially nonmucoid variants were termed "intermediate" in colony morphology. Using DAS-ELISA and Biolog GP MicroPlate SystemTM, mucoid and intermediate variants of Cms were tested to see if these techniques might serve to identify

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GA - Agricultural economics

OECD FORD branch

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Project

<a href="/en/project/GA522%2F00%2F0887" target="_blank" >GA522/00/0887: The use of molecular techniques for understanding of genetic and pathogenic variability in population Clavibacter michiganensis subsp. sepedonicus</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Diseases and Protection

ISSN

1861-3829

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

109

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

630-638

UT code for WoS article

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EID of the result in the Scopus database

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