Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A861" target="_blank" >RIV/00027006:_____/05:861 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus

Original language description

Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA

Czech name

Spolehlivost diagnostických technik pro determinaci Clavibacter michiganensis subsp. sepedonicus

Czech description

Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GA - Agricultural economics

OECD FORD branch

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Project

<a href="/en/project/GA522%2F00%2F0887" target="_blank" >GA522/00/0887: The use of molecular techniques for understanding of genetic and pathogenic variability in population Clavibacter michiganensis subsp. sepedonicus</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Diseases and Protection

ISSN

1861-3829

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

112

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

1-16

UT code for WoS article

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EID of the result in the Scopus database

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