Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A%23%23%23%23%23846" target="_blank" >RIV/00027006:_____/05:#####846 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/05:846

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples

Original language description

Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain(IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were usedfor PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms wasdetermined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliab

Czech name

Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor

Czech description

Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain(IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were usedfor PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms wasdetermined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliab

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GA - Agricultural economics

OECD FORD branch

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Project

<a href="/en/project/QC0048" target="_blank" >QC0048: Molecular and immunoenzymatic diagnosis of selected quarantine, regulated and economical significant plant pathogens.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta fytotechnica et zootechnica

ISSN

1335-258X

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

8

Country of publishing house

SK - SLOVAKIA

Number of pages

4

Pages from-to

5-8

UT code for WoS article

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EID of the result in the Scopus database

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