The use of nutrient semi-selective media for isolation of Clavibacter michiganensis subsp. sepedonicus and detection in ELISA method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A875" target="_blank" >RIV/00027006:_____/04:875 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The use of nutrient semi-selective media for isolation of Clavibacter michiganensis subsp. sepedonicus and detection in ELISA method

Original language description

Clavibacter michiganensis subsp. sepedonicus (Spieckman & Kotthoff) Davis et al. causes the serious ring rot disease of potato in many potato-growing areas around the world. Immunochemical diagnosis of the ring rot bacterium is based on the use of high-quality monoclonal antibodies among several serological methods, of which ELISA belongs to the most important. Although monoclonal antibodies are able to detect very low concentrations of the target bacterium, Cms usually is not found at concentration of103 cells/ml or less. Therefore new techniques and ways are reached to enhance diagnosis of Cms. Commercial monoclonal antibodies (Agdia, USA) were used for determination and detection of Cms in potato tuber extracts by means of DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay) and DASI-ELISA (double antibody sandwich indirect enzyme-linked immunosorbent assay), both with an enrichment step. Four semi-selective media, MTNA, NCP-88, PW 7 and PW 10 were used for cultiv

Czech name

Využití živných semi-selektivních médií pro izolaci Clavibacter michiganensis subsp. sepedonicus a detekci ELISA metodou

Czech description

Clavibacter michiganensis subsp. sepedonicus způsobuje kroužkovitost bramboru. V metodě DASI-ELISA obohacené selektivními médii se ukázaly jako nejvhodnější média MTNA a NCP-88.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F00%2F0887" target="_blank" >GA522/00/0887: The use of molecular techniques for understanding of genetic and pathogenic variability in population Clavibacter michiganensis subsp. sepedonicus</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta fytotechnica et zootechnica

ISSN

1335-258X

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

3

Pages from-to

141-143

UT code for WoS article

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EID of the result in the Scopus database

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