Possibility to detect non-fluid strains of Clavibacter michiganensis subsp. sepedonicus in potato tuber macerate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23911" target="_blank" >RIV/00027006:_____/04:#####911 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Possibility to detect non-fluid strains of Clavibacter michiganensis subsp. sepedonicus in potato tuber macerate

Original language description

Bacterial ring rot disease of potato, caused by Clavibacter michiganensis subsp. sepedonicus (Cms) is a major concern of producers of seed potatoes in the all countries of the world. Current methods of detection of this pathogen rely primarily upon the use of serological procedures including an indirect fluorescent antibody stanining assay (IFAS) and enzyme-linked immunosorbent assay (ELISA). Accurate identification of Cms is especially difficult when population levels are below 10,000 CFU per ml, of plant tissue or millilitre of plant sap. A further complication arises when potato samples contain other bacteria that exhibit cross-reactivity by IFAS and ELISA. Moreover, certain non-fluid variant of Cms that may occur in various natural populations exhibit antigenic variation and escape serological detection. In our tests, potato tubers from samples free of Cms were used. Internal tissue was removed from tubers and homogenized. Cms strains were added to the homogenates to obtain final c

Czech name

Možnost detekce nefluidních kmenů Clavibacter michiganensis subsp. sepedonicus v extraktech z bramborových hlíz

Czech description

Bacterial ring rot disease of potato, caused by Clavibacter michiganensis subsp. sepedonicus (Cms) is a major concern of producers of seed potatoes in the all countries of the world. Current methods of detection of this pathogen rely primarily upon the use of serological procedures including an indirect fluorescent antibody stanining assay (IFAS) and enzyme-linked immunosorbent assay (ELISA). Accurate identification of Cms is especially difficult when population levels are below 10,000 CFU per ml, of plant tissue or millilitre of plant sap. A further complication arises when potato samples contain other bacteria that exhibit cross-reactivity by IFAS and ELISA. Moreover, certain non-fluid variant of Cms that may occur in various natural populations exhibit antigenic variation and escape serological detection. In our tests, potato tubers from samples free of Cms were used. Internal tissue was removed from tubers and homogenized. Cms strains were added to the homogenates to obtain final c

Type

D - Article in proceedings

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

7th Conference of the European Foundation for Plant Pathology & British Society for Plant Pathology Presidential Meeting 2004, University of Aberdeen, UK, 5th x 10th September 2004. Discovery, Development and Delivery in Plant Pathology.

ISBN

není přiřazeno

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

50-50

Publisher name

University os Aberdeen

Place of publication

University of Aberdeen, United Kingdom

Event location

University of Aberdeen, United Kingdom

Event date

Sep 5, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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