Comparison of immunochemical and molecular techniques for reliable detection of fire blight bacterium.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11973" target="_blank" >RIV/60460709:41210/05:11973 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of immunochemical and molecular techniques for reliable detection of fire blight bacterium.

Original language description

Erwinia amylovora [(Burrill) Winslow et al.] (Ea), causing fire blight, is harmful quarantine organism. Fire blight is one of the most serious diseases affecting fruit and ornamental plants of family Rosaceae that has spread in many countries all over the world. Diagnosis of pathogen is based on IFA (indirect fluorescent assay) and bioassay on immature pear fruit assay, but also the other diagnostic techniques are recommended to use for diagnosis of the fire blight organism. PTA-ELISA and IFA, working with polyclonal antibody of Adgen co. (Scotland), were used for testing of 81 isolates of Ea and 38 symptomatic plant samples. Sensitivity of PTA-ELISA, IFA and PCR reached value 106, 105 and 104 CFU/ml, respectively. IFA was not only more sensitive, butalso more specific than PTA-ELISA, because 11 cross-reactions were recorded using IFA in spite of 16 ones in PTA-ELISA. IFA appeared as very sensitive, particularly for screening of plant tissue homogenates. In dilution 1:100 and 1:000, E

Czech name

Porovnání imunochemických a molekulárních technik pro spolehlivou detekci bakterie, způsobující spálu růžovitých rostlin

Czech description

Erwinia amylovora [(Burrill) Winslow et al.] (Ea), causing fire blight, is harmful quarantine organism. Fire blight is one of the most serious diseases affecting fruit and ornamental plants of family Rosaceae that has spread in many countries all over the world. Diagnosis of pathogen is based on IFA (indirect fluorescent assay) and bioassay on immature pear fruit assay, but also the other diagnostic techniques are recommended to use for diagnosis of the fire blight organism. PTA-ELISA and IFA, working with polyclonal antibody of Adgen co. (Scotland), were used for testing of 81 isolates of Ea and 38 symptomatic plant samples. Sensitivity of PTA-ELISA, IFA and PCR reached value 106, 105 and 104 CFU/ml, respectively. IFA was not only more sensitive, butalso more specific than PTA-ELISA, because 11 cross-reactions were recorded using IFA in spite of 16 ones in PTA-ELISA. IFA appeared as very sensitive, particularly for screening of plant tissue homogenates. In dilution 1:100 and 1:000, E

Type

D - Article in proceedings

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

From Laboratory to Business. Recent Advances in Plant Biotechnology. České Budějovice

ISBN

80-86778-16-9

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

87-87

Publisher name

Institute of Plant Molecular Biology ASCR

Place of publication

České Budějovice

Event location

České Budějovice

Event date

Sep 12, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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