Molecular study on acetolactate synthase gene (ALS) of two Czech biotypes of kochia (Kochia scoparia (L.) Schrad.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23777" target="_blank" >RIV/00027006:_____/04:#####777 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular study on acetolactate synthase gene (ALS) of two Czech biotypes of kochia (Kochia scoparia (L.) Schrad.)

Original language description

Two regions of the ALS structural gene known to vary in ALS-resistant biotypes were amplified, digested with restriction endonucleases and one of them sequenced for chlorsulforon-resistant and chlorsulforon-susceptible Kochia scoparia biotypes. The Jihlava resistant kochia biotype contained a single nucleotide substitution in Domain B, predicting a tryptophan (in susceptible) to leucin (in resistant) amino acid change. No other interbiotypic nucleotide differences predicted amino acid changes in the sequenced region, suggesting that the amino acid substitution reported above is responsible for resistance to ALS-inhibiting herbicides in the biotype.

Czech name

Molekulární studie genu acetolaktát syntázy (ALS) dvou českých biotypů bytelu metlatého (Kochia scoparia (L.) Schrad.)

Czech description

Two regions of the ALS structural gene known to vary in ALS-resistant biotypes were amplified, digested with restriction endonucleases and one of them sequenced for chlorsulforon-resistant and chlorsulforon-susceptible Kochia scoparia biotypes. The Jihlava resistant kochia biotype contained a single nucleotide substitution in Domain B, predicting a tryptophan (in susceptible) to leucin (in resistant) amino acid change. No other interbiotypic nucleotide differences predicted amino acid changes in the sequenced region, suggesting that the amino acid substitution reported above is responsible for resistance to ALS-inhibiting herbicides in the biotype.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GA - Agricultural economics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta fytotechnica et zootechnica

ISSN

1335-258X

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

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Country of publishing house

SK - SLOVAKIA

Number of pages

3

Pages from-to

263-265

UT code for WoS article

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EID of the result in the Scopus database

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