Effect of different pretreatment on hop cryopreservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A01%3A_3899" target="_blank" >RIV/00027006:_____/06:01:_3899 - isvavai.cz</a>

Alternative codes found

RIV/14864347:_____/06:01:_3899

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of different pretreatment on hop cryopreservation

Original language description

We compared effect of different pretreatment on hop (Humulus lupulus, L.) cryopreservation. We evaluated impact of 0.75 M sucrose and 60 % polyethylene glycol together with low temperature treatment on survival and regeneration of hop plant. We found outthat pretreatment by both osmotic components had significant effect on an improvement of survival and plant regeneration. The highest plant regeneration was obtained for the method using nodal cuttings pretreatment with 0.75 M sucrose and low temperaturetreatment (2 °C) and following shoot tip loading with 0.7 M sucrose for overnight and air desiccation of shoot tips above silicagel (3 hours). This method is used routinely for cryopreservation of hop germplasm at the Research Institute of Crop Production in Prague in collaboration with the Hop Research Institute in Žatec.

Czech name

Výsledky ruzných predbežných úprav kryoprezervace chmele

Czech description

We compared effect of different pretreatment on hop (Humulus lupulus, L.) cryopreservation. We evaluated impact of 0.75 M sucrose and 60 % polyethylene glycol together with low temperature treatment on survival and regeneration of hop plant. We found outthat pretreatment by both osmotic components had significant effect on an improvement of survival and plant regeneration. The highest plant regeneration was obtained for the method using nodal cuttings pretreatment with 0.75 M sucrose and low temperaturetreatment (2 °C) and following shoot tip loading with 0.7 M sucrose for overnight and air desiccation of shoot tips above silicagel (3 hours). This method is used routinely for cryopreservation of hop germplasm at the Research Institute of Crop Production in Prague in collaboration with the Hop Research Institute in Žatec.

Type

D - Article in proceedings

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/QF3039" target="_blank" >QF3039: Establishing of cryobank of vegetative shoot tips of potato and hop.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

REHOUT Václav (ed.). Biotechnology 2006: 15.-16.2.2006, University of South Bohemia Ceské Budejovice

ISBN

80-85645-53-X

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

558-560

Publisher name

Scientific Pedagogical Publishing

Place of publication

University of South Bohemia Ceské Budejovice

Event location

University of South Bohemia Ceské Budejovice

Event date

Feb 15, 2006

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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