Partial characters of Plum pox virus ´Greek´ and its genetic variability in transgenic Prunus domestica L.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A1486" target="_blank" >RIV/00027006:_____/06:1486 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Partial characters of Plum pox virus ´Greek´ and its genetic variability in transgenic Prunus domestica L.

Original language description

The PPV genome region spanning C-terminal of NIb gene, coat protein (CP) and 3'-non translated region (NCR) gene of a challenging PPV isolate Greek interacting with in transgenic plum has characterized. Different typing assays including ELISA, RT-PCR, RLFP and sequencing (Cter-NIb, CP and 3'NCR region) reveals that challenging PPV-Greek belongs to a typical M subgroup. High sequence (both nucleotide 'nt' and amino acid 'aa') homology were found with M type isolates SK68 (nt 99.0% and aa 100%) or PS (nt98.1% and aa 99.3%) and with recombinant isolate BOR 3 (nt 96.0% and aa 97.4%). The PPV-Greek detected in transgenic plum, resistance clone C-5 or susceptible clone C-6 has shown a few mutations, at the position nt 8441 of Cter-NIb and nt 9179 of CP genein C-6 and at the position nt 9068 in C-5. The mutation in CP gene seems to drive from transgenic transcript, suggest the evidence of template switching between CP cistron of challenging virus and homologous transgenic transcript. These

Czech name

Partial characters of Plum pox virus ´Greek´ and its genetic variability in transgenic Prunus domestica L.

Czech description

The PPV genome region spanning C-terminal of NIb gene, coat protein (CP) and 3'-non translated region (NCR) gene of a challenging PPV isolate Greek interacting with in transgenic plum has characterized. Different typing assays including ELISA, RT-PCR, RLFP and sequencing (Cter-NIb, CP and 3'NCR region) reveals that challenging PPV-Greek belongs to a typical M subgroup. High sequence (both nucleotide 'nt' and amino acid 'aa') homology were found with M type isolates SK68 (nt 99.0% and aa 100%) or PS (nt98.1% and aa 99.3%) and with recombinant isolate BOR 3 (nt 96.0% and aa 97.4%). The PPV-Greek detected in transgenic plum, resistance clone C-5 or susceptible clone C-6 has shown a few mutations, at the position nt 8441 of Cter-NIb and nt 9179 of CP genein C-6 and at the position nt 9068 in C-5. The mutation in CP gene seems to drive from transgenic transcript, suggest the evidence of template switching between CP cistron of challenging virus and homologous transgenic transcript. These

Type

D - Article in proceedings

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník příspěvků z "XVII. české a slovenské konference o ochraně rostlin"

ISBN

80-213-1564-4

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

70-75

Publisher name

ČZU

Place of publication

Praha

Event location

Praha

Event date

Sep 12, 2006

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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