Proliferation and cold hardening of in vitro grown apple shoot tips

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A1736" target="_blank" >RIV/00027006:_____/06:1736 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Proliferation and cold hardening of in vitro grown apple shoot tips

Original language description

Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h ? light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regenera

Czech name

Proliferace a mrazové otužování in vitro kultur jabloní

Czech description

Shoot tips of apple cultivar 'Rubin' were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg L-1 BAP and 0.1 mg L-1 IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg L-1 BAP, which produced 4.2 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. 'Rubin' were cultivated for 4 weeks at low temperature (4°C) and short photoperiod (8/16-h ? light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. In vitro plants were exposed to the controlled cooling rate 4°C/h. The lethal temperature (LT) was -13.0°C for cold hardened and 6.5°C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regenera

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/QC1052" target="_blank" >QC1052: New method of conservation of fruit crop gene resources.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

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Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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