Reliability of molecular and biochemical methods for identification of Pseudomonas syringae and other Pseudomonads recovered from cereals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3A1346" target="_blank" >RIV/00027006:_____/07:1346 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Reliability of molecular and biochemical methods for identification of Pseudomonas syringae and other Pseudomonads recovered from cereals

Original language description

Bacteria Pseudomonas spp. harmful on cereals and grasses were investigated, focusing on Pseudomonas syringae. Fluorescent pseudomonads such as Pseudomonas syringae were found most frequently, but P. fluorescens, P. viridiflava and P. putida were also isolated. Approximately 70% of isolates recovered from plants were identified using microbial identification system BIOLOG BACTERIA to species level, but pathovars of P. syringae were not reliably identified. P. syringae pv. syringae strains were confirmedby serological and HR tests on tobacco. Rep-PCR method was applied to all 24 tested isolates and different genetic profiles were obtained. BOX, ERIC and REP primers were used for amplification of genomic DNA. All three sets of primers gave the same results and allowed discrimination of fingerprints into 7 different genotype groups. Members of these groups were subsequently compared to known profiles of selected type culture strains of several pseudomonads. Two of these groups were positi

Czech name

Spolehlivost molekulárních a biochemických metod pro identifikaci Pseudomonas syringae a dalších pseudomonád z obilnin

Czech description

Byly testovány bakterie Pseudomonas spp.škodící na obilninách a travách. Nejčastěji byly izolovány Pseudomonas syringae pv. syringae, P. fluorescens, P. viridiflava and P. putida

Type

D - Article in proceedings

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník příspěvků "XVII. česká a slovenská konference o ochraně rostlin"

ISBN

80-213-1564-4

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

138-142

Publisher name

ČZU v Praze

Place of publication

Praha

Event location

ČZU, Praha 6 - Suchdol

Event date

Sep 12, 2006

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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