Microarrays and their Aplication in GMO Screening
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3A2485" target="_blank" >RIV/00027006:_____/07:2485 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Microarrays and their Aplication in GMO Screening
Original language description
In this research was developed in-house GMO chip for identification of four plants species, five genetically modified organisms (GMO) and three GMO screening elements. The main goal was to get signal after hybridization of labeled total genomic DNA on the chip. It was tested three ways of labeling templates using fluorescent Cy3/Alexa3 and Cy5/Alexa 5 colours, it was tested hybridization of PCR products, total genomic DNA and treated DNA on the chip. It was determinated sensitivity of developed chip ina way of dilution of hybridization teplate. The value of 25 ng DNA was find as a required amout of DNA, who give a significant signal. However, the signal after hybridization of total genomic DNA wasn?t significant. Thus, we propose another procedures for signal improvement of total genomic DNA.
Czech name
Mikroaraye a jejich aplikace v GMO screeningu.
Czech description
Tento příspěvek popisuje GMO chip pro identifikaci čtyřech rostlinných druhů, s pěti genetickými modifikacemi a tři GMO screeningové elementy. byly testovány tři postupy značení celogenomové DNA.byly použity fluorochromy Cy3/Alexa3 a Cy5/Alexa 5, hybridizace PCR prosuktů. Signálu bylo dosaženo při použití 25ug DNA. Specificita signálu však nebyla dostačující, proto jsme navrhli jiný způsob značení DNA.
Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1B44068" target="_blank" >1B44068: Development and validation of methods for increasing food quality and food safety using molecular biology technologies in agreement with EU regulations.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
4th Advances in Molecular Biology : New Approaches in Plant Analysis
ISBN
978-80-87011-12-6
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
56-61
Publisher name
VÚRV,v.v.i.
Place of publication
Praha
Event location
CR,VÚRV,v.v.i. Praha
Event date
Dec 10, 2007
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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