Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F09%3A00000709" target="_blank" >RIV/00027006:_____/09:00000709 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution

Original language description

The LN33 grapevine plant was artificially infected with Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Although infected with GVB, corky bark symptoms never appeared, and this virus had no destructive effects on the LN33 grapevines under our experimental conditions. Dormant canes, sampled before the beginning of the vegetation period, were used as suitable targets to detectmentioned viruses using both the ELISA and RT-PCR method. For GLRaV-1, the ELISA- based diagnosis unexpectedly appeared more effective than RT-PCR; probably due to the insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in long-term infected grapevine plants showed different degrees of irregularity, depending on the individual viruses.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QG50083" target="_blank" >QG50083: Molecular hybridization for the detection of economically important viruses on grapevine.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Virologica

ISSN

0001-723X

e-ISSN

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Volume of the periodical

53

Issue of the periodical within the volume

4

Country of publishing house

SK - SLOVAKIA

Number of pages

5

Pages from-to

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UT code for WoS article

000272972600010

EID of the result in the Scopus database

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