Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00001757" target="_blank" >RIV/00027006:_____/11:00001757 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/j.1439-0434.2011.01790.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0434.2011.01790.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0434.2011.01790.x" target="_blank" >10.1111/j.1439-0434.2011.01790.x</a>

Result language

angličtina

Original language name

Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction

Original language description

SYBR Green real-time RT PCR assay was developed and optimized for the sensitive detection of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV), Shallot latent virus (SLV) and Mite-borne filamentous virus(MbFV). The polyvalence of the designed primers was tested on 50 genotypes of garlic (Allium sativum L.) which originated from different countries. Plasmid standards were prepared and used as positive standards. The efficiencies of all reactions were 97,93, 99, 98 and 87% for OYDV, LYSV, SLV, GCLV and MbFV standards, respectively. The detection limit for OYDV, LYSV and GCLV was as low as five gene copies, for SLV it was 15 gene copies and for MbFV it was 130 gene copies. In comparison with ELISA, morevirus-positive garlic accessions were detected with LYSV and GCLV by SYBR Green based real-time RT PCR assay. This method was shown to be a more suitable tool for the detection of highly variable pathogens, such as garlic viruses.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QH71228" target="_blank" >QH71228: Optimalization of recovery methods and in vitro propagation for purpose of long-time preservation by cryoconservation of national garlic genotypes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Phytopathology

ISSN

0931-1785

e-ISSN

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Volume of the periodical

159

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

429-434

UT code for WoS article

000289626700006

EID of the result in the Scopus database

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