A new multiplex PCR test for the determination of Vrn-B1 alleles in bread wheat (Triticum aestivum L.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F12%3A00002133" target="_blank" >RIV/00027006:_____/12:00002133 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11032-011-9621-7" target="_blank" >http://dx.doi.org/10.1007/s11032-011-9621-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11032-011-9621-7" target="_blank" >10.1007/s11032-011-9621-7</a>

Result language
angličtina
Original language name
A new multiplex PCR test for the determination of Vrn-B1 alleles in bread wheat (Triticum aestivum L.)
Original language description
In this paper, we describe novel dominant allele Vrn-B1c and new multiplex PCR which we developed. The novel allele contains altered sequence within first intron. It contains both deletion (about 800 bp) and duplication (about 400 bp). Using PCR described in paper, one is able to distinguish four currently known Vrn-B1 alleles (Vrn-B1a, Vrn-B1b, Vrn-B1c and vrn-B1) in one reaction. This PCR vas validated using more than 400 wheat varieties. Novel allele Vrn-B1c was detected in total of 25 spring wheat cultivars.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/OC09033" target="_blank" >OC09033: The study of genes controlling flowering time of wheat eith utilization of specific genetic materials</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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