In‐depth sequence analysis of bread wheat vrn1 genes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00553364" target="_blank" >RIV/61389030:_____/21:00553364 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/21:00554436 RIV/61989592:15310/21:73609382
Result on the web
<a href="http://doi.org/10.3390/ijms222212284" target="_blank" >http://doi.org/10.3390/ijms222212284</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms222212284" target="_blank" >10.3390/ijms222212284</a>
Alternative languages
Result language
angličtina
Original language name
In‐depth sequence analysis of bread wheat vrn1 genes
Original language description
The VERNALIZATION1 (VRN1) gene encodes a MADS‐box transcription factor and plays an important role in the cold‐induced transition from the vegetative to reproductive stage. Allelic variability of VRN1 homoeologs has been associated with large differences in flowering time. The aim of this study was to investigate the genetic variability of VRN1 homoeologs (VRN‐A1, VRN‐B1 and VRN‐D1). We performed an in‐depth sequence analysis of VRN1 homoeologs in a panel of 105 winter and spring varieties of hexaploid wheat. We describe the novel allele Vrn‐B1f with an 836 bp insertion within intron 1 and show its specific expression pattern associated with reduced heading time. We further provide the complete sequence of the Vrn‐A1b allele, revealing a 177 bp insertion in intron 1, which is transcribed into an alternative splice variant. Copy number variation (CNV) analysis of VRN1 homoeologs showed that VRN‐B1 and VRN‐D1 are present in only one copy. The copy number of recessive vrn‐A1 ranged from one to four, while that of dominant Vrn‐A1 was one or two. Different numbers of Vrn‐A1a copies in the spring cultivars Branisovicka IX/49 and Bastion did not significantly affect heading time. We also report on the deletion of secondary structures (G-quadruplex) in promoter sequences of cultivars with more vrn‐A1 copies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA19-05445S" target="_blank" >GA19-05445S: Study of molecular mechanisms of vernalization in wheat</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
22
Issue of the periodical within the volume
22
Country of publishing house
CH - SWITZERLAND
Number of pages
20
Pages from-to
12284
UT code for WoS article
000725873100001
EID of the result in the Scopus database
2-s2.0-85118928059