Validation of the beta-amy1 Transcription Profiling Assay and Selection of Reference Genes Suited for a RT-qPCR Assay in Developing Barley Caryopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F12%3A00002211" target="_blank" >RIV/00027006:_____/12:00002211 - isvavai.cz</a>
Alternative codes found
RIV/25328859:_____/12:#0000654
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0041886" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0041886</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0041886" target="_blank" >10.1371/journal.pone.0041886</a>

Result language
angličtina
Original language name
Validation of the beta-amy1 Transcription Profiling Assay and Selection of Reference Genes Suited for a RT-qPCR Assay in Developing Barley Caryopsis
Original language description
Reverse transcription coupled with real-time quantitative PCR (RT-qPCR) is a frequently used method for gene expression profiling. Reference genes (RGs) are commonly employed to normalize gene expression data. A limited information exist on the gene expression and profiling in developing barley caryopsis. Expression stability was assessed by measuring the cycle threshold (Ct) range and applying both the GeNorm (pair-wise comparison of geometric means) and Normfinder (model-based approach) principles forthe calculation. Here, we have identified a set of four RGs suitable for studying gene expression in the developing barley caryopsis. These encode the proteins GAPDH, HSP90, HSP70 and ubiquitin. We found a correlation between the frequency of occurrenceof a transcript in silico and its suitability as an RG. This set of RGs was tested by comparing the normalized level of beta-amylase (beta-amy1) transcript with directly measured quantities of the BMY1 gene product in the developing barl
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
—

Project
<a href="/en/project/GA521%2F07%2F1028" target="_blank" >GA521/07/1028: Expression analysis of barley beta-amylase gene in relation to sequence differences and grain quality</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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