Invariance of the glass transition temperature of plant vitrification solutions with cooling rate
Result description
The present work endeavours to increase knowledge in this area characterizing the calorimetric glass transition of the most common plant vitrification solutions, under a wide range of cooling rates. The solutions studied were Plant Vitrification Solutions 1, 2 and 3. Cooling was performed using the calorimeter control or for higher rates, by quenching closed pans with PVS in LN, either naked or inside cryovials. Quenched pans were then transferred to the pre-cooled sample chamber. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145 °C to room temperature, at standard warming rate: 10 °C min-1. Glass transitions showed clear and consistent temperature differences among vitrification solutions, related to composition and water content. Roughly, two sets of Tg values were obtained, for PVS1 and 2, at -112 °C and -114 °C, respectively, and for PSV3, at -90 °C. The observed Tg did not significantly change within a wid
Keywords
Cooling ratedifferential scanning calorimetryglass transitionplant vitrification solution
The result's identifiers
Result code in IS VaVaI
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Invariance of the glass transition temperature of plant vitrification solutions with cooling rate
Original language description
The present work endeavours to increase knowledge in this area characterizing the calorimetric glass transition of the most common plant vitrification solutions, under a wide range of cooling rates. The solutions studied were Plant Vitrification Solutions 1, 2 and 3. Cooling was performed using the calorimeter control or for higher rates, by quenching closed pans with PVS in LN, either naked or inside cryovials. Quenched pans were then transferred to the pre-cooled sample chamber. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145 °C to room temperature, at standard warming rate: 10 °C min-1. Glass transitions showed clear and consistent temperature differences among vitrification solutions, related to composition and water content. Roughly, two sets of Tg values were obtained, for PVS1 and 2, at -112 °C and -114 °C, respectively, and for PSV3, at -90 °C. The observed Tg did not significantly change within a wid
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
BJ - Thermodynamics
OECD FORD branch
—
Result continuities
Project
—
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Basic information
Result type
O - Miscellaneous
CEP
BJ - Thermodynamics
Year of implementation
2012