Vitrification Ability of Combined and Single Cryoprotective Agents

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10174608" target="_blank" >RIV/00027006:_____/21:10174608 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2223-7747/10/11/2392/pdf" target="_blank" >https://www.mdpi.com/2223-7747/10/11/2392/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants10112392" target="_blank" >10.3390/plants10112392</a>

Result language

angličtina

Original language name

Vitrification Ability of Combined and Single Cryoprotective Agents

Original language description

Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence. Thermal characteristics and the vitrification ability of two combined CPAs (PVS2 and PVS3), common plant vitrification solutions, and four single CPAs (ethylene glycol, DMSO, glycerol, and sucrose), the components of the mentioned PVSs, were evaluated utilizing a differential scanning calorimetry (DSC) during standard cooling/warming rates of 10 &amp; DEG;C min(-1). The effect of solute concentration on their vitrification ability was shown in the CPAs tested. Four typical concentration regions at which the glassy state and/or crystallization occurred were defined. We suggest the solute concentration of 0.7 g g(-1) as the universal vitrification concentration, characterized by an actual Tg of CPA solution and limited water crystallization. Knowledge of the thermal properties of CPAs allows the design of new combined CPAs with the required vitrification ability respecting the cryopreservation method used and the characteristics of the cryopreserved sample.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910277" target="_blank" >QK1910277: The use of cryopreservation method to streamline breeding process of economically important agricultural crops and preservation of forest tree</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plants-Basel

ISSN

2223-7747

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

2392

UT code for WoS article

000723586200001

EID of the result in the Scopus database

2-s2.0-85118533060