VITRIFICATION PROCESS CONTROL BY DSC

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175408" target="_blank" >RIV/00027006:_____/22:10175408 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

VITRIFICATION PROCESS CONTROL BY DSC

Original language description

Cryopreservation by the vitrification method is based on the prevention of water crystallization and the formation of a glassy state due to treatment with concentrated cryoprotective agents. Both thermal processes, water crystallization / melting and the glass transition can be identified by differential scanning calorimetry (DSC). The optimal composition of the cryoprotectant and the treatment time can be set by measuring the thermal characteristics of the cryopreserved material. DSC measurements can reveal the critical cooling / heating rates or evaluate whether the selected cooling / heating rate can guarantee proper vitrification without harmful ice formation. Diluted plant vitrification solutions PVS2 and PVS3 and their components were measured with TA2920 and Q2000 differential scanning calorimeters (TA Instruments, USA) to identify the safe concentration of the cryoprotectants for their vitrification. The lowest solute concentration suitable for its vitrification started at 0.5 g in 1 g of solution, but the optimal solute concentration approached 0.7 g in 1 g of solution. The &quot;stable&quot; vitrification concentration appeared to be close to 0.8 g in 1 g of solution, when the glass transition temperature corresponded to the Tg&apos;, which characterized to the maximally freeze concentrated solution. Conversely, the presence of Tg&apos; together with the freezing / thawing peak in DSC measurements always reveals ice damage to the sample using the vitrification method. The optimal cryoprotectant composition can be designed to prevent ice crystallization or cryoprotectant toxicity. The thermal characteristics of selected cryoprotectants and explants subjected to the vitrification method measured by DSC will be demonstrated.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910277" target="_blank" >QK1910277: The use of cryopreservation method to streamline breeding process of economically important agricultural crops and preservation of forest tree</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů