Optimization of water content for the cryopreservation of Allium sativum in vitro cultures by encapsulation-dehydration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003731" target="_blank" >RIV/00027006:_____/16:00003731 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Optimization of water content for the cryopreservation of Allium sativum in vitro cultures by encapsulation-dehydration

Original language description

There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. Stem discs were excised from post-harvest, stored bulbs prior to cryopreservation by encapsulation-dehydration and water content was determined gravimetrically. Survival of cryopreserved stem discs was 42.5%, with 22.5% exhibiting shoot regrowth following 6 h desiccation. Gravimetric data demonstrated a correlation between water content corresponding with survival / regrowth from desiccated, cryopreserved stem discs. For encapsulated stem discs a 25% residual moisture and corresponding water content of 0.36 g H2O g-1 d.wt correlated with maximal survival following ~6.5 h of desiccation. The data concurs with the literature suggesting the formation of a stable vitrified state and a ‘window’ for optimal survival and regrowth that is between 6 – 10 h desiccation. Further studies using differential scanning calorimetry (DSC) are suggested to substantiate these findings.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CryoLetters

ISSN

0143-2044

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

308-317

UT code for WoS article

000386541400002

EID of the result in the Scopus database

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