Analysis of freezable water content by DSC for apple dormant bud cryopreservation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005540" target="_blank" >RIV/00027006:_____/19:00005540 - isvavai.cz</a>
Result on the web
<a href="https://www.agriculturejournals.cz/publicFiles/146_2018-HORTSCI.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/146_2018-HORTSCI.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/146/2018-HORTSCI" target="_blank" >10.17221/146/2018-HORTSCI</a>

Result language
angličtina
Original language name
Analysis of freezable water content by DSC for apple dormant bud cryopreservation
Original language description
Dormant bud cryopreservation is a valuable tool for the germplasm of temperate trees preservation. Freezable water content, as an important cryopreservation characteristic, was analysed by DSC. Scions of modern apple tree varieties (Malus domestica) were collected during the winter season from orchards, freeze-dehydrated to 26-32% of water content at -5 degrees C and cryopreserved by the two-step procedure. Samples were rehydrated in moist peat after 4 months of cryopreservation and grafted on rootstocks in a field. Regeneration by sprouting buds on rootstocks was evaluated. The varieties were divided into three groups according to their water content and percentage of crystallized water. The regeneration in the three groups decreased with decreasing bud water content. Regeneration was higher than 80% in most of the varieties and reached 100% in almost 1/3 of the tested varieties. In only one variety, was regeneration lower than 40%, which is still a high regeneration rate for considering apple genotypes as successfully cryopreserved. According to this study, the two-step cryoprotocol was successfully optimized for Central European conditions.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/QJ1630301" target="_blank" >QJ1630301: Development of the new biotechnological systems for maintanance and improvment of agriculture crop and forest tree biodiversitsy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)