Development and validation of RT-PCR multiplex detection of grapevine viruses and viroids in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149401" target="_blank" >RIV/00027006:_____/20:10149401 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs42161-019-00452-x" target="_blank" >https://link.springer.com/article/10.1007%2Fs42161-019-00452-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s42161-019-00452-x" target="_blank" >10.1007/s42161-019-00452-x</a>

Result language
angličtina
Original language name
Development and validation of RT-PCR multiplex detection of grapevine viruses and viroids in the Czech Republic
Original language description
A multiplex RT-PCR reaction was developed and validated for the simultaneous detection of two viruses and two viroids: grapevine fleck virus, grapevine Pinot gris virus, grapevine yellow speckle viroid 1, and hop stunt viroid. The multiplex reaction specificity was verified on a set of 210 grapevines from the Czech Republic. The results showed the occurrence of hop stunt viroid in 94.3% of the examined plants, while incidence of the other pathogens was lower: grapevine Pinot gris virus (64.8%), grapevine yellow speckle viroid 1 (52.4%), and grapevine fleck virus (15.2%).
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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