Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome-induced pathways

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177171" target="_blank" >RIV/00027006:_____/24:10177171 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10478355

Result on the web

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/pmic.202300312" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/pmic.202300312</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pmic.202300312" target="_blank" >10.1002/pmic.202300312</a>

Result language

angličtina

Original language name

Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome-induced pathways

Original language description

The ectoparasitic mite Varroa destructor transmits and triggers viral infections that have deleterious effects on honey bee colonies worldwide. We performed a manipulative experiment in which worker bees collected at emergence were exposed to Varroa for 72 h, and their proteomes were compared with those of untreated control bees. Label-free quantitative proteomics identified 77 differentially expressed Apis mellifera proteins (DEPs). In addition, viral proteins were identified by orthogonal analysis, and most importantly, Deformed wing virus (DWV) was found at high levels/intensity in Varroa-exposed bees. Pathway enrichment analysis suggested that the main pathways affected included peroxisomal metabolism, cyto-/exoskeleton reorganization, and cuticular proteins. Detailed examination of individual DEPs revealed that additional changes in DEPs were associated with peroxisomal function. In addition, the proteome data support the importance of TGF-beta signaling in Varroa-DWV interaction and the involvement of the mTORC1 and Hippo pathways. These results suggest that the effect of DWV on bees associated with Varroa feeding results in aberrant autophagy. In particular, autophagy is selectively modulated by peroxisomes, to which the observed proteome changes strongly corresponded. This study complements previous research with different study designs and suggests the importance of the peroxisome, which plays a key role in viral infections.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910018" target="_blank" >QK1910018: The development of MULTIOMICS pesticide risk assessment for bees with regard to real contamination, cocktail effect, and other stressors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PROTEOMICS

ISSN

1615-9853

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

2300312

UT code for WoS article

001180416500001

EID of the result in the Scopus database

2-s2.0-85187190650