Elimination of Black raspberry necrosis virus from raspberry (Rubus idaeus L.) by in vitro cryotherapy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177368" target="_blank" >RIV/00027006:_____/24:10177368 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/24:00601740

Result on the web

<a href="https://www.pubhort.org/ejhs/89/3/16/89_3_16.pdf" target="_blank" >https://www.pubhort.org/ejhs/89/3/16/89_3_16.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/eJHS.2024/016" target="_blank" >10.17660/eJHS.2024/016</a>

Result language

angličtina

Original language name

Elimination of Black raspberry necrosis virus from raspberry (Rubus idaeus L.) by in vitro cryotherapy

Original language description

This study aims to establish a cryopreservation protocol for in vitro grown raspberry (Rubus idaeus L.) without the use of dimethyl sulfoxide (DMSO). The protocol exploits cryotherapy for virus eradication in selected favourable genotypes and will facilitate safe preservation of raspberry genotypes in the Czech national cryobank. Raspberries are highly valuable perennial berries grown in temperate countries including the Czech Republic. Raspberry cultivars and elite breeding lines must be maintained as plant collections either in the field or as in vitro cultures. Both maintenance methods are suboptimal primarily because of the many viral and other pathogens transmitted in raspberry field collections, the industriousness, the maintenance costs, and possible somaclonal variation associated with maintenance of in vitro cultures. For these reasons, cryopreservation is currently considered as a prospective method for the long-term preservation of germplasm. One of the best-performing vitrification procedures for plant cryopreservation is based on DMSO solutions. However, due to the potentially mutagenic effect of DMSO, which is contradictory to the principle of germplasm preservation, we focused on testing a DMSO-free- free procedure using two raspberry varieties. Regrowth rate of the raspberry variety &apos;Tulameen&apos; after application of Plant Vitrification Solution 3 (PVS3) followed by immersion in liquid nitrogen (LN) was 86%. For the raspberry variety Autumn First, the regrowth level was 73%. In vitro shoots of the cryopreserved variety &apos;Tulameen&apos; were multiplied in a standard cultivation medium and RT-PCR screened for eradication of Black raspberry necrosis virus (BRNV). The eradication rate of BRNV from in vitro cultures of &apos;Tulameen&apos; by cryopreservation was 94%. The eradication of BRNV from control samples, not subjected to LN, was not significantly different (92%). It can be concluded that the DMSO-free- free tested method is suitable for efficient cryoconservation. Although the LN phase of the cryoprotocol is not required, the PVS3 protocol alone represents a valuable tool for eradication of BRNV from in vitro cultures of tested raspberry shoots. The suggested protocol will be used for the safe backup of healthy plant material.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/TO01000295" target="_blank" >TO01000295: Healthy berries in a changing climate: development of new biotechnological procedures for virus diagnostics, vector studies, elimination and safe preservation of strawberry and raspberry</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE

ISSN

1611-4426

e-ISSN

—

Volume of the periodical

89

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

eJHS2024016

UT code for WoS article

001332714700002

EID of the result in the Scopus database

2-s2.0-85210443526