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Effects of genistein and genistin on in vitro maturation of pig oocytes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F08%3A%230000800" target="_blank" >RIV/00027014:_____/08:#0000800 - isvavai.cz</a>

  • Alternative codes found

    RIV/60460709:41210/08:25732

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effects of genistein and genistin on in vitro maturation of pig oocytes

  • Original language description

    The effects of GEN (genistein ? an inhibitor of tyrosine protein kinase ? TPK) and GIN (genistin, an analogue of GEN without effects on TPK) on pig oocyte maturation and cumular cell expansion under in vitro conditions and connection with their estrogenic activity were tested. Further, the reversibility of GEN effects was verified. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities. As for GIN, with an increase in its concentration, the number of oocytes blocked at the GV stage significantly decreased, but the abnormal maturation increased. GEN inhibited the cumular cell expansion in proportion to its dose. GIN had a less pronounced effect. As GEN and GIN effects demonstrate similar patterns, it is probable that estrogenic activity is involved.

  • Czech name

    Vliv genisteinu a genistinu na dozrávání prasečích oocytů in vitro

  • Czech description

    Byly testovány vlivy genisteinu (GEN ? inhibitor protein tyrozin kinázy ? TPK) a GIN (genistein ? glykosid GEN bez bez inhibičních účinků na TPK) na dozrávání prasečích oocytů a expanzi kumulárních buněk v podmínkách in vitro a souvislost s jejich estrogenní aktivitou. Dále byla ověřována reverzibilita vlivů GEN. V závislosti na koncentraci blokoval GEN dozrávání prasečích oocytů až do stádia zárodečného váčku (GV). Po vypláchnutí GEN se dozrávání oocytů obnovilo, ale docházelo k abnormalitám. U GIN sepři zvyšování koncentrace počet oocytů blokovaných ve stádiu GV průkazně snížil, ale případů abnormálního dozrávání přibylo. GEN potlačoval růst kumulárních buněk úměrně ke své dávce. Vliv GIN byl méně patrný. GEN i GIN působily podobně, a proto je pravděpodobné, že estrogenní aktivita se na jejich účincích podílí.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GD523%2F03%2FH076" target="_blank" >GD523/03/H076: Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Czech Journal of Animal Science

  • ISSN

    1212-1819

  • e-ISSN

  • Volume of the periodical

    53

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    8

  • Pages from-to

  • UT code for WoS article

    000253027900001

  • EID of the result in the Scopus database