Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F10%3A%230001310" target="_blank" >RIV/00027014:_____/10:#0001310 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes

Original language description

Porcine sperm heads were injected into mouse ovulated and in vitro matured oocytes. Both pronuclei were formed after injection into mouse ovulated oocytes. Additional labelling against 5-MeC and some histone modification revealed similarity to those changes that are typical for mouse zygotes. After porcine sperm head injection into in vitro matured mouse oocytes, no paternal pronuclei were formed. In the other part of the study, mouse sperm head were injected into mouse in vitro matured oocytes. The labelling against 5-MeC showed less extensive demethylation than is typical for ovulated oocytes. Our observation suggest that porcine sperm heads are capable of undergoing active demethylation in vivo matured mouse oocytes and that in vitro matured mouse oocytes posses lower sperm remodeling capabilities.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Reproduction and Development

ISSN

0916-8818

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

6

Country of publishing house

JP - JAPAN

Number of pages

6

Pages from-to

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UT code for WoS article

000285859300006

EID of the result in the Scopus database

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