Dynamics of epigenetic remodeling in interspecies porcine zygotes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001575" target="_blank" >RIV/00027014:_____/12:#0001575 - isvavai.cz</a>
Result on the web
<a href="http://www.agriculturejournals.cz/publicFiles/58327.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/58327.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Dynamics of epigenetic remodeling in interspecies porcine zygotes
Original language description
The process of active paternal chromatin demethylation after fertilization in the pig is not fully understood and very inconsistent data have been published by different research groups. We have applied the interspecies intracytoplasmic sperm injection (iICSI) to evaluate remodeling capabilities of porcine oocytes in more details. We injected mouse frozen-thawed sperm heads into porcine in vitro matured or ovulated oocytes, respectively. Embryos produced by intracytoplasmic sperm injection (ICSI) of boar spermatozoa into porcine ovulated oocytes (intraspecies) served as controls. Zygotes with 2-pronuclei were labeled with antibodies against certain epigenetic modifications (5-methylcytosine, 5-MeC, heterochromatin protein 1, HP1, trimethylation of H3/K4, H3/K4-me3, and dimethylation of H3/K9, H3/K9-me2). The labeling patterns were not different between zygotes produced from in vitro matured and ovulated oocytes. Both pronuclei were symmetrically labeled with 5-MeC, HP1, and H3/K4-me3 a
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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