Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F18%3AN0000037" target="_blank" >RIV/00027014:_____/18:N0000037 - isvavai.cz</a>

Result on the web

<a href="https://vuzv.cz/_privat/18037.pdf" target="_blank" >https://vuzv.cz/_privat/18037.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development to term of sheep embryos reconstructed after inner cell mass/trophoblast exchange

Original language description

Here we report in vitro and term development of sheep embryos after the inner cell mass (ICM) from one set of sheep blastocysts were injected into the trophoblast vesicles of another set. We also observed successful in vitro development of chimeric blastocysts made from sheep trophoblast vesicles injected with bovine ICM. First, we dissected ICMs from 35 sheep blastocysts using a stainless steel microblade and injected them into 29 re-expanded sheep trophoblastic vesicles. Of the 25 successfully micromanipulated trophoblastic vesicles, 15 (51.7%) re-expanded normally and showed proper ICM integration. The seven most well reconstructed embryos were transferred for development to term. Three ewes receiving manipulated blastocysts were pregnant at day 45 (42.8%), and all delivered normal offspring (singletons, two females and one male, average weight: 3.54 ± 0.358 kg). Next, we monitored in vitro development of sheep trophoblasts injected with bovine ICMs. Of 17 injected trophoblastic vesicles, 10 (58.8%) re-expanded after 4 h in culture, and four (40%) exhibited integrated bovine ICM. Our results indicate that ICM/trophoblast exchange is feasible, allowing full term development with satisfactory lambing rate. Therefore, ICM exchange is a promising approach for endangered species conservation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Reproduction and Development

ISSN

0916-8818

e-ISSN

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Volume of the periodical

64

Issue of the periodical within the volume

2

Country of publishing house

JP - JAPAN

Number of pages

5

Pages from-to

187-191

UT code for WoS article

000430524500011

EID of the result in the Scopus database

2-s2.0-85045395745